SEMINAL ATTRIBUTES, COOLING PROCEDURE AND POST THAW QUALITY OF SEMEN OF INDIGENOUS KHARI BUCK OF NEPAL

Abstract

The objective of the study was to understand the seminal attributes, effectiveness of cooling process and post thawed semen quality of a Nepalese indigenous Khari buck. Two Khari bucks were used for the study. Thirty-two ejaculates (16 ejaculates from each buck) were evaluated for seminal attributes of fresh semen. The mean semen volume, semen color, sperm mass activity, sperm motility, sperm viability, sperm concentration, abnormal acrosome, mid piece and tail, and abnormal head were 0.73 ml, 3.10, 3.80, 80.9%, 94.6%, 2597.0×106 ml-1 , 10.7% and 5.00%, respectively. Further, 12 ejaculates (6 ejaculates) from each buck (only having ≥0.5ml semen volume, ≥80% sperm motility, ≥90% sperm viability and ≥2500×106 ml-1 sperm concentration) were considered for cooling, freezing and post thawed semen quality. Tris-egg-yolk[1]citrate extender was used for semen dilution. The mean sperm motility and viability of post diluted semen after 90 minutes of cooling were 73.8% and 88.1%, respectively. Similarly, the mean sperm motility and viability of post diluted semen after 210 minutes of cooling were 69.2% and 85.0%, respectively. The mean post thaw sperm motility and viability were 49.0% and 81.2%, respectively. The study concluded that the seminal attributes and results of post thaw semen quality are acceptable and indicated the feasibility of cryopreserving Khari buck semen.